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transblot turbo semidry transfer system  (Bio-Rad)


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    Bio-Rad transblot turbo semidry transfer system
    Transblot Turbo Semidry Transfer System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13624 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transblot+turbo+semidry+blotting+system/Trans-Blot+Turbo+Transfer+System/10__1161_slash_jaha__125__042248-217-16-21
    Average 99 stars, based on 13624 article reviews
    transblot turbo semidry transfer system - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Systemic administration of the OGT inhibitor OSMI-1 normalizes hippocampal O-GlcNAcylation and improves recognition memory, redox balance, and brain mitochondrial homeostasis in a Rett syndrome mouse model.
    Article Snippet: The gel was UV-activated using Image Lab Software (Bio-Rad Laboratories) to capture images of the total protein load. .. Following electrophoresis and gel imaging, proteins were transferred onto nitrocellulose membranes (#162-0115, Bio-Rad Laboratories) using the TransBlot Turbo semidry blotting system (#1704150, Bio-Rad Laboratories). .. The membranes were blocked with 3 % bovine serum albumin in 0.5 % Tween-20/Tris-buffered saline (TTBS) and incubated overnight at 4 ◦C with the following primary antibodies: O-GlcNAc CTD110.6 (1:500; SC-59623, Santa Cruz Biotechnology), O-GlcNAc RL2 (1:1000; MABS157, Sigma‒Aldrich), OGT (1:500; SC-74546, Santa Cruz Biotechnology), OGA (1:1000; SAB 4200267, Sigma-Aldrich), pS243GFAT1 (1:1000; S343C, MRC-PPU), GFAT1 (1:1000; 28121, IBL), pT172AMPK (1:1000; GTX52341, GeneTex), AMPKα1/2 (1:500; SC-74461, Santa Cruz Biotechnology), CaMKII (1:1000; ab175459, Abcam), LKB1 (1:1000; 3047, Cell Signaling Technology), pS428LKB1 (1:1000; 3482, Cell Signaling Technology), HO-1 (1:1000; ADI-SPA-895-F, Enzo Life Sciences), NQO1 (1:1000; 15926, Invitrogen/Thermo Fisher Scientific), OXPHOS (1:3000; Ab011413, Abcam), PGC-1α (1:1000; sc-518025, Santa Cruz Biotechnology), NRF1 (1:500; MA5-32782, Invitrogen), mt-TFA (1:500; sc-166965, Santa Cruz Biotechnology), VDAC1 (1:500; sc-390996, Santa Cruz Biotechnology), SOD2 (1:500; sc-137254, Santa Cruz Biotechnology), pS616DRP1 (1:1000; #3455, Cell Signaling Technology) and pS637DRP1 (1:1000; #4867, Cell Signaling Technology).

    Imaging:

    Article Title: Systemic administration of the OGT inhibitor OSMI-1 normalizes hippocampal O-GlcNAcylation and improves recognition memory, redox balance, and brain mitochondrial homeostasis in a Rett syndrome mouse model.
    Article Snippet: The gel was UV-activated using Image Lab Software (Bio-Rad Laboratories) to capture images of the total protein load. .. Following electrophoresis and gel imaging, proteins were transferred onto nitrocellulose membranes (#162-0115, Bio-Rad Laboratories) using the TransBlot Turbo semidry blotting system (#1704150, Bio-Rad Laboratories). .. The membranes were blocked with 3 % bovine serum albumin in 0.5 % Tween-20/Tris-buffered saline (TTBS) and incubated overnight at 4 ◦C with the following primary antibodies: O-GlcNAc CTD110.6 (1:500; SC-59623, Santa Cruz Biotechnology), O-GlcNAc RL2 (1:1000; MABS157, Sigma‒Aldrich), OGT (1:500; SC-74546, Santa Cruz Biotechnology), OGA (1:1000; SAB 4200267, Sigma-Aldrich), pS243GFAT1 (1:1000; S343C, MRC-PPU), GFAT1 (1:1000; 28121, IBL), pT172AMPK (1:1000; GTX52341, GeneTex), AMPKα1/2 (1:500; SC-74461, Santa Cruz Biotechnology), CaMKII (1:1000; ab175459, Abcam), LKB1 (1:1000; 3047, Cell Signaling Technology), pS428LKB1 (1:1000; 3482, Cell Signaling Technology), HO-1 (1:1000; ADI-SPA-895-F, Enzo Life Sciences), NQO1 (1:1000; 15926, Invitrogen/Thermo Fisher Scientific), OXPHOS (1:3000; Ab011413, Abcam), PGC-1α (1:1000; sc-518025, Santa Cruz Biotechnology), NRF1 (1:500; MA5-32782, Invitrogen), mt-TFA (1:500; sc-166965, Santa Cruz Biotechnology), VDAC1 (1:500; sc-390996, Santa Cruz Biotechnology), SOD2 (1:500; sc-137254, Santa Cruz Biotechnology), pS616DRP1 (1:1000; #3455, Cell Signaling Technology) and pS637DRP1 (1:1000; #4867, Cell Signaling Technology).



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